SARS-CoV-2 Nucleocapsid Protein Antibody na Neutralizing Antibody Combo Ngwa ngwa ule.

SARS-CoV-2 Nucleocapsid Protein Antibody na Neutralizing Antibody Combo Nnwale ngwa ngwa egosipụtara

SARS-CoV-2 Nucleocapsid protein Antibody na Neutraliz-ing Antibody Comb Test Rapid

Ụdị:Mpempe akwụkwọ anaghị ebipụ

Akara:Bio-mapper

Katalọgụ:RS101601

Ụdị:WB/S/P

Mmetụta:97.60%

Nkọwapụta:99.40%

Nnwale serological siri ike iji chọpụta ọgwụ mgbochi na-egbochi SARS-CoV-2 dị mkpa ngwa ngwa iji chọpụta ọ bụghị naanị ọnụego ọrịa, mgbochi anụ ụlọ na amụma ihu ọchị, kamakwa ịdị irè ọgwụ mgbochi n'oge ule ụlọ ọgwụ na mgbe ịgba ọgwụ mgbochi buru ibu.

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Nkọwa zuru ezu

Tụlee ihe ọ bụla sitere na mmadụ ka ọ na-efe efe ma jikwaa ya site na iji usoro biosafety ọkọlọtọ.

Plasma

1. Chịkọta ụdị ọbara n'ime lavender, acha anụnụ anụnụ ma ọ bụ akwụkwọ ndụ akwụkwọ ndụ top collection tube (nwere EDTA, citrate ma ọ bụ heparin, karị na Vacutainer®) site na veinpuncture.

2.Kewaa plasma site na centrifugation.

3.Crefully ịdọrọ plasma n'ime ọhụrụ tupu akara tube.

Serum

1. Chịkọta ụdị ọbara n'ime tube mkpokọta na-acha uhie uhie (nke enweghị ọgwụ mgbochi ọbara na Vacutainer®) site na veinpuncture.

2.Kwe ka ọbara kụọ.

3. Kewaa serum site na centrifugation.

4.Crefully ịdọrọ serum n'ime a ọhụrụ akpa akara tube.

5.Test ụdị ngwa ngwa o kwere omume mgbe ịnakọta.Na-echekwa ụdịdị na 2°C ruo 8°C ma ọ bụrụ na anwaleghị ya ozugbo.

6.Na-echekwa ụdịdị na 2°C ruo 8°C ruo ụbọchị 5.A ga-ekporịrị ụdịdị ahụ na -20 Celsius maka nchekwa ogologo oge

Ọbara

Enwere ike nweta ntụpọ nke ọbara dum site na nkuchi aka n'ọnụ mkpịsị aka ma ọ bụ veinpuncture.Ejila ọbara hemolaized ọ bụla maka nnwale.Ekwesịrị ịchekwa ụdị ọbara niile na friji (2°C-8°C) ma ọ bụrụ na anwaleghị ya ozugbo.A ghaghị ịnwale ihe nlere ahụ n'ime awa 24 nke nchịkọta. Zenarị ọtụtụ okirikiri oyi-ajụ oyi.Tupu anwale, weta ụdị oyi kpọnwụrụ n'ime ụlọ ahụ nwayọ wee gwakọta nwayọ.A ga-akọwapụta ihe nlere nwere obere ihe a na-ahụ anya site na centrifugation tupu nnwale.

Usoro ASsay

Kwụpụ 1: Weta ihe nlere ahụ wee nwalee ihe mejupụtara ya n'ime ụlọ ma ọ bụrụ na etinyere ya na friji ma ọ bụ friji.Ozugbo agbazere, gwakọta ihe nlele ahụ nke ọma tupu ị nyocha.

Kwụpụ 2: Mgbe ị dị njikere ịnwale, mepee obere akpa na notch wee wepụ ngwaọrụ.Tinye ngwaọrụ nnwale n'elu ala dị ọcha ma dịkwa larịị.

Kwụpụ 3: Jide n'aka na ị ga-eji akara njirimara kanye ngwaọrụ ahụ.

Kwụpụ 4: Maka ule ọbara dum - Tinye 1 dobe ọbara dum (ihe dịka 30-35 µL) n'ime nlele nke ọma.- Mgbe ahụ tinye 2 tụlee (ihe dị ka 60-70 µL) nke Sample Diluent ozugbo.