NKỌWA NA NKWAWA NWA Nnwale
Leptospirosis na-apụta n'ụwa niile ma bụrụkwa nsogbu ahụike dị nro na nke siri ike maka ụmụ mmadụ na anụmanụ, ọkachasị na mpaghara ihu igwe na-ekpo ọkụ na iru mmiri.Ebe nchekwa ebumpụta ụwa maka leptospirosis bụ òké yana nnukwu anụ ụlọ dị iche iche.Ọrịa mmadụ na-ebute site na L. interrogans, onye na-ahụ maka ọrịa nke Leptospira.A na-ekesa ọrịa ahụ site na mmamịrị sitere na anụ ụlọ.
Mgbe ọrịa ahụ gasịrị, leptospires na-adị n'ime ọbara ruo mgbe a kpochapụrụ ha mgbe ụbọchị 4 ruo 7 gasịrị na mmepụta nke anti-L.interrogans ọgwụ mgbochi ọrịa, nke mbụ nke klas IgM.Omenala nke ọbara, mmamịrị na mmiri cerebrospinal bụ ụzọ dị irè iji kwado nchoputa ahụ n'ime izu 1st ruo nke abụọ mgbe ekpughere ya.Nchọpụta serological nke antiL.interrogans ọgwụ mgbochi ọrịa bụkwa usoro a na-achọpụtakarị.Ule dị n'okpuru udi a: 1) Nnwale agglutination microscopic (MAT);2) ELISA;3) Nnwale mgbochi fluorescent na-apụtaghị ìhè (IFAT).Agbanyeghị, usoro niile a kpọtụrụ aha n'elu chọrọ ụlọ ọrụ ọkaibe na ndị ọkachamara zụrụ nke ọma.
Leptospira IgG/IgM bụ nnwale serological dị mfe nke na-eji antigens sitere na L. interrogans wee chọpụta ọgwụ mgbochi IgG na IgM na microorganisms ndị a n'otu oge.Enwere ike ịme ule a site n'aka ndị ọrụ na-azụghị ọzụzụ ma ọ bụ ndị nwere obere nkà, na-enweghị ngwa ụlọ nyocha na-akpa ike na nsonaazụ dị n'ime nkeji iri na ise.
Ụkpụrụ
Ngwa ule ngwa ngwa Leptospira IgG/IgM bụ chromatographic na-erugharị n'akụkụ
immunoassay.Kaseti ule ahụ nwere: 1) pad conjugate nwere agba burgundy nwere recombinant L. interrogans antigens conjugated na colloid gold (Leptospira conjugates) na oke bekee IgG-gold conjugates, 2) nitrocellulose akpụkpọ ahụ warara nwere abụọ ule bands (M na G bands) na otu njikwa njikwa (C band).Ejiri M bandeji kpuchie ya na monoclonal mgbochi mmadụ IgM maka nchọpụta mgbochi L.. interrogans IgM, G band bu ụzọ kpuchie ya na reagents maka nchọpụta mgbochi L.gbara ajụjụ ọnụ IgG, na ndị otu C ejirila ewu mgbochi oke bekee IgG kpuchie ya.
Mgbe a na-ekesa oke nlere ule zuru oke n'ime olulu mmiri nke cassette ahụ, ihe atụ ahụ na-akwaga site na capillary edinam gafee cassette.IgM anti-L.. ndị na-ajụ ajụjụ ma ọ bụrụ na ha dị na ụdị ga-ejikọta Leptospira conjugates.A na-ejidekwa immunocomplex na akpụkpọ ahụ ahụ site na mgbochi IgM mgbochi mmadụ kpuchiri ya, na-akpụ otu agba agba burgundy, na-egosi nsonaazụ L.. interrogans IgM ziri ezi.IgG anti-L.. ndị na-ajụ ajụjụ ma ọ bụrụ na ha dị na ụdị ga-ejikọta Leptospira conjugates.A na-ejidekwa immunocomplex ahụ site na reagents nwere mkpuchi mkpuchi mbụ na akpụkpọ ahụ, na-akpụ otu agba agba burgundy G, na-egosi nsonaazụ nyocha nke L.. interrogans IgG.
Enweghị eriri ule ọ bụla (M na G) na-egosi nsonaazụ adịghị mma.Nnwale ahụ nwere njikwa ime (C band) nke kwesịrị igosi eriri agba agba burgundy nke immunocomplex nke ewu mgbochi oke bekee IgG/oke bekee IgG-gold conjugate n'agbanyeghị mmepe agba na ụdọ ule ọ bụla.Ma ọ bụghị ya, nsonaazụ ule abaghị uru yana a ga-eji ngwaọrụ ọzọ nwaleghachi ihe nlele ahụ.
