Nkọwa zuru ezu
Nchọpụta nje nje atụrụ pox bụ microplate ejirila protein antigen nke atụrụ pox kpuchie ya, ihe nrịbama enzyme na ihe ndị ọzọ na-akwado reagents, na ụkpụrụ nke immunoassay njikọ enzyme-linked (ELISA) na-eji iji chọpụta mgbochi atụrụ pox n'ime ahụ. atụrụ serum sample.N'oge nnwale ahụ, a na-agbakwunye serum njikwa na ihe nlele a ga-enyocha na efere microplate, ma ọ bụrụ na ihe nlele ahụ nwere ọgwụ mgbochi atụrụ mgbe emechara ya, a ga-ejikọta ya na antigen na efere microplate, yana ihe ndị ọzọ na-adịghị ejikọta. a ga-ewepụ mgbe ịsachara;Mgbe ahụ gbakwunye ihe nrịbama enzyme ahụ ka ọ jikọta kpọmkwem na mgbagwoju antigen-antibody na efere microplate;A na-ewepụ ihe nrịbama enzyme ahụ na-adịghị agwụ agwụ site na ịsacha, ma tinye ihe ngwọta TMB n'olulu mmiri, na ngwaahịa na-acha anụnụ anụnụ bụ mmeghachi omume nke microplate conjugates mepụtara, na omimi agba na-ejikọta ya na ọnụ ọgụgụ ọgwụ mgbochi dị na ya. sample.Mgbe agbakwunyere ngwọta nkwụsị ahụ iji kwụsị mmeghachi omume ahụ, ngwaahịa ahụ gbanwere edo edo;Onye na-agụ microplate na-ekpebi uru nnabata dị na mmeghachi omume ọ bụla nke ọma n'ogo nke 450 nm iji chọpụta ma ihe nlele ahụ ọ nwere ọgwụ nje pox atụrụ.
Ọdịnaya ahaziri ahazi
Usoro nrụpụta ngwa ngwa akwụkwọ mpempe akwụkwọ anaghị ebipụ
